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Windows 2003 server to Windows 2016 Server Upgrade , RDS ISSUES

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Dear all,

I have two Windows Server 2003 as Domain controllers with replication working very well, as they are old, i decided to upgrade them to Windows 2016 Server by creating two new windows server 2016 machine and add them into the domain as domain controller (upgrade forest functionnal level from 2003 to 2008)

For now, 2003 server are still serving and replicating to Windows 2016 servers, so now i have 4 domain controllers.The FSMO roles are still spread accross the two 2003 DCs, so the 2016 DCs are just here but that's all.

I have an RDS server which is hosting some apps, and i had the following issue, which is now solved (https://www.mysysadmintips.com/windows/servers/505-the-remote-desktop-license-server-cannot-update-event-4105).

Another issue is now present and i have problems regarding solving it.

That issue is that users who wants to log to the RDS app obtain an "acces denied" when trying to login.

If i disconnect the two 2016 DCs, the RDS login is working....

Do somebody have any idea about that issue ?Is there a version problem ?2K16 not compatible with  RDS servers which are 2008 R2.. ?I'm totally stuck :(

Many thanks at all for your help !



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