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LDAP failures Server '08 R2

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Hey anyone,

My place of work has been having some troubles with authenticating users for an off-site program that uses our domain credentials. We have a single Active Directory domain consisting of just two DC's. One DC is a physical box and the other is a virtual machine running on VMWare (both Windows Server 2008R2). 

When credentials are put in for this program, they are passed from the workstation to a server that hosts the program. The server then runs a check against our domain controllers to authenticate the users. This is the point where everything stops. Through some testing we have found that if we take our virtual DC offline, our physical DC will go ahead and answer the queries and therefor authenticate the user. When we turn the virtual DC back on, we find that BOTH reply to the query simultaneously with an ldap failure.

We have tried to think about what the real issue is, whether it be a misconfiguration or a networking error, but cannot seem to come up with a real solution. If anyone can shed some light on the situation or give me some more ideas it would be deeply appreciated. We are tired of putting bandages over bandages to make things work.


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